ROCK2 Rabbit Monoclonal Antibody

Western Blot analysis using Hela cell lysate, Proteins were separated by 4-20% SDS-PAGE

Western Blot analysis using Hela cell lysate, Proteins were separated by 4-20% SDS-PAGE

Catalog Number:MABN95166
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml. The concentration of this product may be batch-dependent.
Host:Rabbit
Isotype:IgG
Immunogen:The specific immunogen used to produce this antibody is proprietary information.
Reactivity:Human,Mouse,Rat
Applications:WB 1:2000-1:10000; IF-P/IF-F/IF-ICC 1:200-1:1000; ELISA 1:5000-1:20000
Molecular Weight:Observed MW: 161 kDa; Calculated MW: 161 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:ROCK2; KIAA0619; Rho-associated protein kinase 2; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164ROCK-2

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Applications

WB 1:2000-1:10000; IF-P/IF-F/IF-ICC 1:200-1:1000; ELISA 1:5000-1:20000

Immunogen

The specific immunogen used to produce this antibody is proprietary information.

Target Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Synonyms:ROCK2; KIAA0619; Rho-associated protein kinase 2; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164ROCK-2

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

PBS, 50% glycerol, 0.05% Proclin300, 0.05% protective protein

Western Blot analysis using Hela cell lysate, Proteins were separated by 4-20% SDS-PAGE

Immunohistochemistry analysis of paraffin-embedded Rat brain using ROCK2 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ROCK2 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

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