S1PR1

Western blot using S1PR1 on extracts of
various cell lines at 1:1000 dilution.
Secondary antibody: 
HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk inTBST.
Detection: ECL West Pico Kit.  
Exposure time: 90s.

Western blot using S1PR1 on extracts of various cell lines at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL West Pico Kit. Exposure time: 90s.

Catalog Number:AB-83739
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant funsion protein containing a sequence of human S1PR1
Reactivity:Hu, Ms
Applications:Western Blot: 1:500- 1:1000 Immunofluorescence: 1:200-1:1,000
Molecular Weight:43kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CD363;CHEDG1;D1S3362;ECGF1;EDG-1;EDG1;S1P1

Applications

Western Blot: 1:500- 1:1000 Immunofluorescence: 1:200-1:1,000

Immunogen

Recombinant funsion protein containing a sequence of human S1PR1

Target Background

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants.

Synonyms:CD363;CHEDG1;D1S3362;ECGF1;EDG-1;EDG1;S1P1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot using S1PR1 on extracts of various cell lines at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL West Pico Kit. Exposure time: 90s.

Immunofluorescence: 1:200 Immunofluorescence analysis of A549 cells, using S1P Receptor EDG1 Antibody. The picture on the right is blocked with the synthesized peptide

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