SAM68

Western blot analysis of extracts of various cell lines, using SAM68 antibody ) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL west Pico Plus.   
Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using SAM68 antibody ) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL west Pico Plus. Exposure time: 10s.

Catalog Number:AB-84326
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the Cterminus of human SAM68.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:68kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:KHDRBS1;Sam68;p62;p68

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300 to the Cterminus of human SAM68.

Target Background

This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants.

Synonyms:KHDRBS1;Sam68;p62;p68

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using SAM68 antibody ) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL west Pico Plus. Exposure time: 10s.

Immunohistochemistry of paraffin embedded rat brain using SAM68 antibody at dilution of 1:100 (40x lens).

Confocal immunofluorescence analysis of HeLa cells using SAM68Polyclonal Antibody at dilution of 1:400. Blue: DAPI for nuclear staining.

Review for SAM68 Rabbit pAb Experimen Type: Immunofluorescence(IF) Sample: Zebrafish retina Description: Immunofluorescence analysis of Zebrafish retina, using SAM68 Polyclonal Antibody.

Review for SAM68 Rabbit pAb Experiment Type: Immunofluorescence(IF) Sample: Zebrafish retina Description: Immunofluorescence analysis of Zebrafish retina, using PCBP2 Polyclonal Antibody.

No documents available.