Immunological Sciences

SHP-1 Rabbit Polyclonal Antibody

Western Blot analysis of 3T3 cells using SH-PTP1 Polyclonal Antibody

Western Blot analysis of 3T3 cells using SH-PTP1 Polyclonal Antibody

Catalog Number:AB-84762
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human SHP-1. AA range:502-551
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1:50-200 ELISA: 1/20000
Molecular Weight:67kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatograp
Form:Liquid
Synonyms:PTPN6; HCP; PTP1C; Tyrosine-protein phosphatase non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosinephosphatase SHP-1; SH-PTP1

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1:50-200 ELISA: 1/20000

Immunogen

The antiserum was produced against synthesized peptide derived from human SHP-1. AA range:502-551

Target Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

Synonyms:PTPN6; HCP; PTP1C; Tyrosine-protein phosphatase non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosinephosphatase SHP-1; SH-PTP1

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of 3T3 cells using SH-PTP1 Polyclonal Antibody

Western blot analysis of lysates from NIH/3T3 cells, using SHP-1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SHP-1 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of mouse spleen using SHP1 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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