Immunological Sciences

SIRT1

Western blot analysis of extracts from normal (control) and SIRT1 knockout HeLa cells, using SIRT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of extracts from normal (control) and SIRT1 knockout HeLa cells, using SIRT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-82681
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:130kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SIR2;SIR2L1;SIR2alpha;SIRT1;sirtuin 1

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1.

Target Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Synonyms:SIR2;SIR2L1;SIR2alpha;SIRT1;sirtuin 1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts from normal (control) and SIRT1 knockout HeLa cells, using SIRT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using SIRT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody. Western blot was performed from the immunoprecipitate using SIRT1 antibody at a dilition of 1:1000.

Immunohistochemistry of paraffin embedded human colon using SIRT1 Rabbit pAb at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using SIRT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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