Immunological Sciences

SLC7A11 / xCT Rabbit Polyclonal Antibody

Western blot analysis of lysates from wild type (WT) and SLC7A11/xCT knockdown (KD) HeLa cells using [KD Validated] SLC7A11/xCT Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of lysates from wild type (WT) and SLC7A11/xCT knockdown (KD) HeLa cells using [KD Validated] SLC7A11/xCT Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-84812
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SLC7A11/xCT.
Reactivity:Human,Mouse,Rat
Applications:Western Blot:     1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay  requirements.
Molecular Weight:42kDa
Purification:Affinity purification
Form:Liquid
Synonyms:xCT; CCBR1; SLC7A11/xCT

Applications

Western Blot:     1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay  requirements.

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SLC7A11/xCT.

Target Background

This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcomaassociated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death.

Synonyms:xCT; CCBR1; SLC7A11/xCT

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of lysates from wild type (WT) and SLC7A11/xCT knockdown (KD) HeLa cells using [KD Validated] SLC7A11/xCT Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

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