SLUG

Western blot analysis of extracts of various cell lines, using SLUG antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Kit
Exposure time: 60s..

Western blot analysis of extracts of various cell lines, using SLUG antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit Exposure time: 60s..

Catalog Number:AB-84208
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 89-268 of human SLUG
Reactivity:Hu, Ms, Rt
Applications:Western Blot : 1:500-1:2,000 Immunofluorescence/Immunocytochemistry: 1:50-1: 200 Immunohistochemsitry (paraffin, formalin, frozen Tissues): 1:50-1:200
Molecular Weight:29kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SNAI2; SLUG; SLUGH; SLUGH1; SNAIL2; WS2D

Applications

Western Blot : 1:500-1:2,000 Immunofluorescence/Immunocytochemistry: 1:50-1: 200 Immunohistochemsitry (paraffin, formalin, frozen Tissues): 1:50-1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 89-268 of human SLUG

Target Background

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Synonyms:SNAI2; SLUG; SLUGH; SLUGH1; SNAIL2; WS2D

Storage

Storage for short term at 4°C recommended, for longer term at -20°C, minimize freeze/thaw cycles

Buffer

PBS with 0,02% sodium azide, 50% glycerol pH7.3

Western blot analysis of extracts of various cell lines, using SLUG antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit Exposure time: 60s..

Immunohistochemistry of paraffin-embedded rat brain using SLUG Antibody at dilution of 1:100

Application: Western blot (WB) Sample mouse cells Loading Amount 15 ug Dilution 1:1000 Incubation time over night Temperature : 4 ℃ Exposure: 30s Observed Band: 25 kDa

Application: Western blot Sample MDA-MB-231 Loading Amount 15 ug Dilution 1:2000 Incubation time over night Temperature 4 °C Exposure 30s Observed Band 29 kDa

Immunofluorescence analysis of A549 cells using SLUG antibody. Blue: DAPI for nuclear staining.

No documents available.