Smad2/3
Western blot analysis of extracts of HeLa cells, using SMAD2/SMAD3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s.
| Catalog Number: | AB-90212 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human SMAD3. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100 |
| Molecular Weight: | 56-60kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3 |
Applications
Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human SMAD3.