Smoothened Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using SMO antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 120s.

Western blot analysis of extracts of various cell lines, using SMO antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 120s.

Catalog Number:AB-84784
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-233 of human SMO.
Reactivity:Human, Mouse
Applications:Western Blot: 1:100 - 1:500
Molecular Weight:86kDa
Purification:Affinity purification
Form:Liquid
Synonyms:Gx; CRJS; PHLS; SMOH; FZD11; SMO

Applications

Western Blot: 1:100 - 1:500

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 28-233 of human SMO.

Target Background

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Synonyms:Gx; CRJS; PHLS; SMOH; FZD11; SMO

Storage

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Buffer

Store at -20℃. Avoid freeze / thaw cycles.

Western blot analysis of extracts of various cell lines, using SMO antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 120s.

Western blot analysis of extracts of Mouse brain, using SMO antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico plus. Exposure time: 180s.

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