SNAIL

Western blot analysis of extracts of
various cell lines, using SNAIL
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.  
Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using SNAIL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-84195
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human SNAIL
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:34kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SNAI1;SLUGH2;SNA;SNAH;SNAIL;SNAIL1;dJ710H13.1

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human SNAIL

Target Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.

Synonyms:SNAI1;SLUGH2;SNA;SNAH;SNAIL;SNAIL1;dJ710H13.1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using SNAIL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Immunohistochemistry of paraffin embedded rat kidney using SNAIL antibody at dilution of 1:100 (40x lens).

Review for SNAIL Polyclonal Antibody Application: Western blot (WB) Sample: mouse cells Loading: Amount15 ug Dilution: 1:1000 Incubation: timeover night Temperature: 4 ℃ Exposure: 30s Observed Band: 35 kDa Remarks: Western blot analysis of extracts of mouse cells, using antibody at 1:1000 dilution.

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