Immunological Sciences

Somatostatin Rabbit Polyclonal Antibody

Western Blot analysis of A549 cells using Somatostatin Polyclonal Antibody

Western Blot analysis of A549 cells using Somatostatin Polyclonal Antibody

Catalog Number:AB-81812
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Somatostatin. AA range:10-59.
Reactivity:Human;Mouse;Rat
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/100 -1/200
Molecular Weight:17kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:SST; Somatostatin; Growth hormone release-inhibiting factor

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/100 -1/200

Immunogen

The antiserum was produced against synthesized peptide derived from human Somatostatin. AA range:10-59.

Target Background

The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells.

Synonyms:SST; Somatostatin; Growth hormone release-inhibiting factor

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of A549 cells using Somatostatin Polyclonal Antibody

Western Blot analysis of various cells using Somatostatin Polyclonal Antibody

Western blot analysis of lysates from 1) PC-12, 2) A549 ,3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7)

Immunohistochemical analysis of paraffin-embedded Human Gastric fundus. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunofluorescence analysis of paraffinembedded rat pancreas using Somatostatin (SST) Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffinembedded mouse pancreas using Somatostatin (SST) Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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