Immunological Sciences

SOX2

Figure 1: Western blot analysis using SOX2 mAb against HEK293 (1) and SOX2-hIgGFc transfected HEK293 (2) cell lysate.

Figure 1: Western blot analysis using SOX2 mAb against HEK293 (1) and SOX2-hIgGFc transfected HEK293 (2) cell lysate.

Catalog Number:MAB-90964
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Ms
Isotype:IgG1
Clone:10F10
Immunogen:Purified recombinant fragment of human SOX2 expressed in E. Coli.
Reactivity:Hu
Applications:Western Bloting: 1/250 - 1/1000. Immunohistochemistry: 1/100 - 1/500. Immunofluorescence: 1/100 - 1/500. ELISA: Propose dilution 1/5000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
Molecular Weight:Calculated MW: 34kDa Observed MW: ~65kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Bloting: 1/250 - 1/1000. Immunohistochemistry: 1/100 - 1/500. Immunofluorescence: 1/100 - 1/500. ELISA: Propose dilution 1/5000. Not yet tested in other applications. Determining optimal working dilutions by titration test.

Immunogen

Purified recombinant fragment of human SOX2 expressed in E. Coli.

Target Background

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Storage

Store at 4°, for long term storage, store at -20°.

Buffer

Ascitic fluid containing 0.03% sodium azide.

Figure 1: Western blot analysis using SOX2 mAb against HEK293 (1) and SOX2-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis of NTERA-2 cells using SOX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and esophageal cancer tissues (right) using SOX2 mouse mAb with DAB staining

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