Immunological Sciences

SOX2

1. SOX2 Immunohistochemistry Frozen Tissues dilution 1:100 on cortex tissues of wild type mouse “enlargement 40x 03”

1. SOX2 Immunohistochemistry Frozen Tissues dilution 1:100 on cortex tissues of wild type mouse “enlargement 40x 03”

Catalog Number:AB-83387
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant protein of human SOX2
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500- 1:1000Immunohistochemistry (Paraffin-embedded tissues): 1:50 - 1:100Immunohistochemistry (Frozen Tissues) 1:100- 1:200Immunocytochemsitry: 1:100-1:200 Immunofluorescence: 1:100- 1:200
Molecular Weight:34kDa
Purification:Aff. Pur.
Form:Liquid
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Applications

Western Blot: 1:500- 1:1000Immunohistochemistry (Paraffin-embedded tissues): 1:50 - 1:100Immunohistochemistry (Frozen Tissues) 1:100- 1:200Immunocytochemsitry: 1:100-1:200 Immunofluorescence: 1:100- 1:200

Immunogen

Recombinant protein of human SOX2

Target Background

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

1. SOX2 Immunohistochemistry Frozen Tissues dilution 1:100 on cortex tissues of wild type mouse “enlargement 40x 03”

2. SOX2 Immunohistochemistry Frozen Tissues dilution: 1:100 on striated tissues of wild type mouse “enlargement 63x”

3. Western blot analysis of brain cell lysate using SOX2 antibody.

4. Immunohistochemistry of paraffin-embedded rat brain using SOX2 antibody at dilution of 1:100 (400x lens).

5. Immunohistochemistry of paraffin-embedded mouse brain using SOX2 antibody at dilution of 1:100 (400x lens).

6. Immunofluorescence analysis of MCF7 cell using SOX2 antibody. Blue: DAPI for nuclear staining.

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