SOX2 Mouse Monoclonal Antibody

Western blot analysis using SOX2 mouse mAb against C6(1),F9(2) cell lysate.

Western blot analysis using SOX2 mouse mAb against C6(1),F9(2) cell lysate.

Catalog Number:MABN80930
Conjugate:Unconjugated
Size:100ul
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Immunogen:Purified recombinant fragment of human SOX2 expressed in E. Coli.
Reactivity:Human,Mouse,Rat,Rabbit,Monkey
Applications:WB 1:500-1:1000,IHC 1:200-1:1000,ICC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400
Molecular Weight:34kDa
Purification:Affinity purification
Form:liquid
Synonyms:ANOP3; MCOPS3

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Applications

WB 1:500-1:1000,IHC 1:200-1:1000,ICC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400

Immunogen

Purified recombinant fragment of human SOX2 expressed in E. Coli.

Target Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Synonyms:ANOP3; MCOPS3

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide.

Western blot analysis using SOX2 mouse mAb against C6(1),F9(2) cell lysate.

Immunofluorescence analysis of NTERA-2 cells using SOX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence analysis of NIH3T3 cells using SOX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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