SOX9

Western blot analysis of extracts of
various cell lines, using SOX9
antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus. 
Exposure time: 3min.

Western blot analysis of extracts of various cell lines, using SOX9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Catalog Number:MAB-94563
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:S9
Immunogen:A synthesized peptide derived from human SOX9.
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:70kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CMD1;CMPD1;SRA1;SRXX2;SRXY10;SOX9;SRY-box 9

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthesized peptide derived from human SOX9.

Target Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Synonyms:CMD1;CMPD1;SRA1;SRXX2;SRXY10;SOX9;SRY-box 9

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using SOX9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Immunohistochemistry of paraffinembedded rat testis using SOX9 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffinembedded mouse testis using SOX9 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded human colon using SOX9 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SOX9 antibody. Blue: DAPI for nuclear staining.

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