SOX9 Rabbit Monoclonal Antibody

Western blot analysis of lysates from HepG2 cells, using SOX9 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Western blot analysis of lysates from HepG2 cells, using SOX9 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Catalog Number:MABN21543
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG,Kappa
Immunogen:Recombinant protein of human SOX9
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:200-1:1000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000
Molecular Weight:Observed MW:70kD
Purification:Protein A
Form:Liquid

Cell localization:Nucleus.SRY-box 9(SOX9) Homo sapiens The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

Applications

WB 1:500-1:1000,IHC 1:200-1:1000,ICC/IF 1:200-1:1000,ELISA 1:5000-1:20000

Immunogen

Recombinant protein of human SOX9

Target Background

Cell localization:Nucleus.SRY-box 9(SOX9) Homo sapiens The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein

Western blot analysis of lysates from HepG2 cells, using SOX9 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using SOX9 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using SOX9 Rabbit mAb at a dilution of 1:1000 (40xlens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

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