Immunological Sciences

SQSTM1/ P62

Western blot analysis of extracts of various cell lines, using 62/SQSTM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit. Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using 62/SQSTM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit. Exposure time: 10s.

Catalog Number:AB-83779
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human P62/SQSTM1
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200
Molecular Weight:62kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human P62/SQSTM1

Target Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Storage

Store at -20℃. Avoid freeze / thaw cycles

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using 62/SQSTM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit. Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 30s.

Immunohistochemistry of paraffin embedded human colon carcinoma using SQSTM1 / p62 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded human tonsil using SQSTM1 / p62 antibody at dilution of 1:150 (40x lens).

Immunofluorescence analysis of HeLa cells using SQSTM1 / p62 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using SQSTM1 / p62 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

No documents available.