Stat6 (phospho Tyr641) Rabbit Polyclonal Antibody

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],function:Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.,PTM:Tyrosine phosphorylated following stimulation by IL-4 and IL-3.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member (By similarity). Interacts with NCOA1 via its C-terminal LXXLL motif.,

Applications

WB 1:500-1:2000,IHC 1:100-1:300,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000

Immunogen

Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using STAT6 （Phospho-Tyr641） Antibody Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT6 （Phospho-Tyr641） Antibody. The picture on the right is blocked with the phospho peptide. Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT6 （Phospho-Tyr641） Antibody. The lane on the right is blocked with the phospho peptide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.