Immunological Sciences

SUMO1 Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-84756
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-101 of human Sumo 1.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:17kDa/80kDa
Purification:Affinity purification
Form:Liquid
Synonyms:DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-101 of human Sumo 1.

Target Background

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.

Synonyms:DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3

Storage

Store at -20℃. Avoid freeze / thaw cycles..

Buffer

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using SUMO1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1min.

Western blot analysis of extracts from wild type(WT) and SUMO1 knockout (KO) 293T cells, using SUMO1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded human breast cancer using SUMO1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Confocal imaging of NIH/3T3 cells using [KO Validated] SUMO1 Rabbit pAb (dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

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