SUMO3 Rabbit Polyclonal antibody

Western blot analysis of extracts of various cell lines, using SUMO3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.   
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using SUMO3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-84758
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human SUMO3.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:17-50kDa
Purification:Affinity purification
Form:Liquid
Synonyms:SMT3A; Smt3B; SMT3H1; SUMO-3

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human SUMO3.

Target Background

This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described.

Synonyms:SMT3A; Smt3B; SMT3H1; SUMO-3

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.01% thiomersal,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using SUMO3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Immunofluorescence analysis of U-2 OS cells using SUMO3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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