Synaptophysin

Western blot analysis of extracts of various cell lines, using Synaptophysin  antibody
at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus 
Exposure time: 20s.

Western blot analysis of extracts of various cell lines, using Synaptophysin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 20s.

Catalog Number:AB-82642
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Synaptophysin.
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200
Molecular Weight:38kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:SYP; MRX96; MRXSYP;

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Synaptophysin.

Target Background

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).

Synonyms:SYP; MRX96; MRXSYP;

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using Synaptophysin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 20s.

Immunohistochemistry of paraffin embedded rat brain using Synaptophysin antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse spinal cord using Synaptophysin antibody at dilution of 1:100 (40x lens).

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