Immunological Sciences

TAF15

Western blot analysis of extracts of various cell lines, using TAF15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s.

Western blot analysis of extracts of various cell lines, using TAF15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s.

Catalog Number:AB-84381
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280of human TAF15
Reactivity:Hu
Applications:WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:200 IP: 1:50 - 1:200
Molecular Weight:80kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:TAF15;Npl3;RBP56;TAF2N;TAFII68

Applications

WB: 1:500 - 1:2000 IHC: 1:50 - 1:100 IF: 1:50 - 1:200 IP: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280of human TAF15

Target Background

This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Synonyms:TAF15;Npl3;RBP56;TAF2N;TAFII68

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using TAF15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s.

Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug TAF15 antibody. Western blot was performed from the immunoprecipitate using TAF15 antibody at a dilition of 1:1000.

Immunohistochemistry of paraffinembedded human liver cancer using TAF15 Rabbit pAb at dilution of 1:150 (40x lens).

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