Immunological Sciences

TDP43 Rabbit Polyclonal Antibody

Western blot analysis of various lysates using TDP-43/TARDBP Rabbit pab at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of various lysates using TDP-43/TARDBP Rabbit pab at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-83805
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-400 of human TDP-43/TARDB.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 ChIP: 1:50 - 1:200
Molecular Weight:35kDa/45kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:ALS10; TDP-43; TDP-43/TARDBP

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 ChIP: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human TDP-43/TARDB.

Target Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Synonyms:ALS10; TDP-43; TDP-43/TARDBP

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3..3.

Western blot analysis of various lysates using TDP-43/TARDBP Rabbit pab at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Chromatin immunoprecipitation analysis of extracts of K562 cells, using TDP-43/TARDBP Rabbit pAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Confocal imaging of HeLa cells using TDP-43/TARDBP Rabbit pAb dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse pAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded rat ovary using TDP-43/TARDBP Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded human esophageal using TDP-43/TARDBP Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded mouse brain using TDP-43/TARDBP Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of TDP-43/TARDBP in paraffin-embedded rat spleen using TDP-43/TARDBP Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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