TGN38

Western blot analysis of extracts of HeLa cells, using TGN38 Rabbit mAb  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus 
Exposure time: 1s.

Western blot analysis of extracts of HeLa cells, using TGN38 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 1s.

Catalog Number:MAB-94619
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:TG21
Immunogen:A synthesized peptide derived from human TGN46
Reactivity:Hu
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:90-100kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:TGN38; TGN46; TGN48; TGN51; TTGN2

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthesized peptide derived from human TGN46

Target Background

This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Synonyms:TGN38; TGN46; TGN48; TGN51; TTGN2

Storage

Store for short term at 4-8°C .At -20°C for longer term. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of HeLa cells, using TGN38 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 1s.

Immunohistochemistry of paraffin Embedded human liver using TGN38 Rabbit mAb at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-2 OS cells using TGN38 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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