Immunological Sciences

Thrombospondin-1/THBS1

Western blot analysis of extracts of various cell lines, using THBS1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using THBS1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Catalog Number:AB-83786
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-360 of human THBS1
Reactivity:Hu, Ms,
Applications:Western Blot: 1:1000
Molecular Weight:150kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:THBS;THBS-1;TSP;TSP-1;TSP1

Applications

Western Blot: 1:1000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-360 of human THBS1

Target Background

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cellto- matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.

Synonyms:THBS;THBS-1;TSP;TSP-1;TSP1

Storage

Store for short term at 4-8°C .At -20°C for longer term. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using THBS1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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