Immunological Sciences

TNF alpha Rabbit Polyclonal Antibody

Western blot analysis of THP-1, using TNF-α Rabbit pAb at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of THP-1, using TNF-α Rabbit pAb at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-90222
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1/500 - 1/2000 Immuohistochemistry(paraffin-embedded tissues) 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000
Molecular Weight:16kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Applications

Western Blot: 1/500 - 1/2000 Immuohistochemistry(paraffin-embedded tissues) 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000

Immunogen

The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190.

Target Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Synonyms:TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of THP-1, using TNF-α Rabbit pAb at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western Blot analysis of various cells using TNF-α Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunohistochemistry of paraffinembedded human colon carcinoma using TNF-α Rabbit pAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded human liver using TNF-α Rabbit pAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded human spleen using TNF-α Rabbit pAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of A549. 1,primaryAntibody(red) was diluted at 1:200(4°C overnight). 2, GoatAnti Rabbit IgG (H&L) - Alexa Fluor 594 Secondaryantibody was diluted at 1:1000(room temperature, 50min). 3, Picture B: DAPI(blue) 10min.

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