TNF alpha Polyclonal Antibody

Western Blot analysis of Jurkat cells with TNF alpha Polyclonal Antibody

Western Blot analysis of Jurkat cells with TNF alpha Polyclonal Antibody

Catalog Number:ABE3623
Conjugate:Unconjugated
Size:100ug
Concentration:1 mg/mL
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:Synthesized peptide derived from the Internal region of human TNF-α
Reactivity:Human;Mouse;Rat
Applications:Western Blot: 1:500-1:2000; Immunohistochemistry: 1:100-1:300;Immunofluorescence: 1:200-1:1000
Molecular Weight:16 kDa
Purification:Affinity purification
Synonyms:TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Applications

Western Blot: 1:500-1:2000; Immunohistochemistry: 1:100-1:300;Immunofluorescence: 1:200-1:1000

Immunogen

Synthesized peptide derived from the Internal region of human TNF-α

Target Background

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Synonyms:TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha

Storage

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Buffer

Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.

Western Blot analysis of Jurkat cells with TNF alpha Polyclonal Antibody

Immunofluorescence analysis of Human stomach tissue using TNF alpha Polyclonal Antibody at dilution of 1:200.

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