TOM20

Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.  
Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:MAB-94609
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:EPR15581-54
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1).
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:20 - 1:50 Immunoprecipitation: 1:50 - 1:200
Molecular Weight:16kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:TOMM20;MAS20;MOM19;TOM20

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:20 - 1:50 Immunoprecipitation: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1).

Target Background

Synonyms:TOMM20;MAS20;MOM19;TOM20

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using TOM20 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TOM20 antibody. Western blot was performed from the immunoprecipitate using TOM20 antibody at a dilution of 1:1000.

Immunohistochemistry of paraffin embedded human colon using TOM20 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using TOM20 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Review for TOM20 Rabbit mAb Experiment Type Immunofluorescence (IF) Sample: Mouse brown fat cells

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