TOMM20 Rabbit Monoclonal Antibody

Western blot analysis of lysates from HepG2 cells, using TOMM20 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Western blot analysis of lysates from HepG2 cells, using TOMM20 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Catalog Number:MABN21541
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG,Kappa
Clone:Monoclonal
Immunogen:Recombinant protein of human TOMM20
Reactivity:Human,Mouse,Rat
Applications:WB 1:1000-1:5000;IHC 1:100-1:500;IF 1:200-1:1000;ELISA 1:5000-1:20000
Molecular Weight:Observed MW:16kD
Purification:Protein A
Form:Liquid
Synonyms:TOMM20;KIAA0016;Mitochondrial import receptor subunit TOM20 homolog;Mitochondrial 20 kDa outer membrane protein;Outer mitochondrial membrane receptor Tom20

Cell localization:Membranous.function:Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with tom22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the tom40 translocation pore.,similarity:Belongs to the Tom20 family.,subunit:Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22.

Applications

WB 1:1000-1:5000;IHC 1:100-1:500;IF 1:200-1:1000;ELISA 1:5000-1:20000

Immunogen

Recombinant protein of human TOMM20

Target Background

Cell localization:Membranous.function:Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with tom22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the tom40 translocation pore.,similarity:Belongs to the Tom20 family.,subunit:Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22.

Synonyms:TOMM20;KIAA0016;Mitochondrial import receptor subunit TOM20 homolog;Mitochondrial 20 kDa outer membrane protein;Outer mitochondrial membrane receptor Tom20

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%protective protein

Western blot analysis of lysates from HepG2 cells, using TOMM20 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue. 1, TOMM20 Rabbit Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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