Transferrin

Western blot analysis of extracts of various cell lines, using Transferrin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus
Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using Transferrin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 30s.

Catalog Number:AB-83950
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 399-698 of human Transferrin.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500- 1:000 Immunohistochemistry: 1:50 -1:00 Immunofluorescence: 1:50 - 1:00
Molecular Weight:77kDa
Purification:Purified
Form:Liquid
Synonyms:TF; HEL-S-71p; PRO1557; PRO2086; TFQTL1; transferrin

Applications

Western Blot: 1:500- 1:000 Immunohistochemistry: 1:50 -1:00 Immunofluorescence: 1:50 - 1:00

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 399-698 of human Transferrin.

Target Background

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.

Synonyms:TF; HEL-S-71p; PRO1557; PRO2086; TFQTL1; transferrin

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using Transferrin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 30s.

No documents available.