Immunological Sciences

TRKA

Western blot analysis of extracts of various cell lines, using TrkA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 10s

Western blot analysis of extracts of various cell lines, using TrkA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 10s

Catalog Number:AB-83821
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TrkA
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry:1:50 - 1:200
Molecular Weight:120 kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry:1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TrkA

Target Background

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

Synonyms:MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Storage

Store at -20℃. Avoid freeze / thaw cycles

Buffer

PBS with 0.02% sodium azide,50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using TrkA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 10s

Immunohistochemistry of paraffinembedded mouse stomach using TrkA Antibody at dilution of 1:100 (40xlens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded rat cerebellum using TrkA Antibody at dilution of 1:100 (40xlens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol

No documents available.