TUBB3 Mouse Monoclonal Antibody

Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.

Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.

Catalog Number:MABN80763
Conjugate:Unconjugated
Size:100ul
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Clone:Monoclonal
Immunogen:Purified recombinant fragment of human TUBB3 expressed in E. Coli.
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:2000,ICC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400
Molecular Weight:55kDa
Purification:Affinity purification
Form:liquid
Synonyms:tubulin, beta 3; MC1R; TUBB4

Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, a- and

Applications

WB 1:500-1:2000,ICC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400

Immunogen

Purified recombinant fragment of human TUBB3 expressed in E. Coli.

Target Background

Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, a- and

Synonyms:tubulin, beta 3; MC1R; TUBB4

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

PBS containing 0.03% sodium azide.

Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.

Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of A549 cells using TUBB3 mouse mAb (green) and negative control (purple).

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