Tubulin Beta Mouse Monoclonal Antibody

WB analysis of beta Tubulin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.

WB analysis of beta Tubulin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.

Catalog Number:MABS15140
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG1, κ
Clone:TUB2
Immunogen:Recombinant protein corresponding to Human beta Tubulin
Reactivity:Species, Human, Mouse, Rat
Applications:Applications Dilution<br/>WB 1: 1000-1: 2000<br/>ICC/IF 1: 800-1: 1600
Purification:Affinity purification
Form:Liquid
Synonyms:Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain,β tubulin, CDCBM6

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Applications

Applications Dilution<br/>WB 1: 1000-1: 2000<br/>ICC/IF 1: 800-1: 1600

Immunogen

Recombinant protein corresponding to Human beta Tubulin

Target Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Synonyms:Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain,β tubulin, CDCBM6

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

WB analysis of beta Tubulin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Mouse lgG , 1: 5000, RT, 1h.

ICC analysis of beta Tubulinred.Sample: HeLa, 4% PFA 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG , 1: 300, RT, 1h.

ICC analysis of beta Tubulinred.Sample: NIH3T3, 4% PFA 20min.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 1000, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG , 1: 300, RT, 1h.

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