Immunological Sciences

Tubulin Beta Rabbit Monoclonal Antibody

WB analysis of beta Tubulin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 5000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of beta Tubulin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 5000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

Catalog Number:MABS152667
Conjugate:unconjugated
Size:200 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG, κ
Clone:TUB02
Immunogen:Recombinant protein corresponding to Human beta Tubulin
Reactivity:Species, Human, Mouse, Rat
Applications:Applications Dilution<br/>WB 1: 5000-1: 10000<br/>ICC/IF 1: 100-1: 300
Purification:Affinity purification
Form:Liquid
Synonyms:Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain,β tubulin, CDCBM6
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Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Applications

Applications Dilution<br/>WB 1: 5000-1: 10000<br/>ICC/IF 1: 100-1: 300

Immunogen

Recombinant protein corresponding to Human beta Tubulin

Target Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Synonyms:Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain,β tubulin, CDCBM6

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

WB analysis of beta Tubulin.Sample: Protein treated by RIPA Lysis Buffer .Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h.Primary antibody: 1: 5000, 4℃ overnight.Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

ICC analysis of beta Tubulinred.Sample: C6, were fixed in pre-cooled methanol 10 minutes.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 100, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

ICC analysis of beta Tubulinred.Sample: C6, were fixed in pre-cooled methanol 10 minutes.Blocking buffer: 3% BSA in PBS, RT, 30min.Primary antibody: 1: 300, 4℃ overnight.Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG , 1: 300, RT, 1h.

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