Immunological Sciences

Tubulin betaIII

Western Blot analysis using Tubulin Beta III Monoclonal Antibody.

Western Blot analysis using Tubulin Beta III Monoclonal Antibody.

Catalog Number:MAB-10288
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Ms
Isotype:IgG1
Clone:TU-20
Immunogen:Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Reactivity:Hu, Ms, Ch, Bv, Pig, Rt, Ha, Fh
Applications:­ Flow Cytometry ­ Western Blotting Recommended dilution:1-2 µg/ml, 90 min Positive control: Porcine brain lysate Negative control: HPB-ALL human peripheral blood leukemia cell line Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. ­ Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/ml Staining technique: Standard ABC technique (DAB+) Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue ­ Immunocytochemistry Positive material: Neuro2a mouse neuroblastoma cell line
Purification:Aff. Pur.
Form:Liquid

Applications

­ Flow Cytometry ­ Western Blotting Recommended dilution:1-2 µg/ml, 90 min Positive control: Porcine brain lysate Negative control: HPB-ALL human peripheral blood leukemia cell line Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. ­ Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/ml Staining technique: Standard ABC technique (DAB+) Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue ­ Immunocytochemistry Positive material: Neuro2a mouse neuroblastoma cell line

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Target Background

The tubulin beta III isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human beta III-tubulin.

Storage

Store at 2-8°C. Do not freeze.

Buffer

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Western Blot analysis using Tubulin Beta III Monoclonal Antibody.

Immunohistochemistry staining of human brain (paraffin sections) using anti-betaIII tubulin (TU-20).

Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 μg/ml). Nuclei were stained with DAPI (blue).

Immunocytochemistry staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). B - Merged image of co-staining with anti-beta-tubulin (TU-06; green; cat. no. 11-251-C100). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).

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