Immunological Sciences

TWIST1 Rabbit Monoclonal Antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution .

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution .

Catalog Number:MABN36693
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG,Kappa
Clone:Monoclonal
Immunogen:Endogenous
Reactivity:Human,Mouse, Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000
Molecular Weight:26 kDa
Purification:Protein A
Form:liquid
Synonyms:BHLHA38 TWIST

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000

Immunogen

Endogenous

Target Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

Synonyms:BHLHA38 TWIST

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% Protective protein

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃, over night with a 1:5000 dilution .

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TWIST1 antibody. The HRP Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Immunofluorescence analysis of HeLa . Picture A: TWIST1 Rabbit mAb (red). Picture B: DAPI (blue). Picture C: Merge of A+B

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