β-Actin Mouse mAb
Western blot analysis of lysates from HeLa cells, using β-Actin Mouse mAb (AC004) at 1:2000- 1:640000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
| Catalog Number: | AB004 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG1,Kappa |
| Clone: | AMC0001 |
| Immunogen: | Fusion protein of human β-Actin |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB,1:5000-1:160000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 42kDa |
| Purification: | Affinity purification |
| Form: | liquid |
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Applications
WB,1:5000-1:160000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Fusion protein of human β-Actin
