β-Actin Rabbit mAb
Western blot analysis of various lysates, using β-Actin Rabbit mAb at 1:120000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
| Catalog Number: | AB048 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC5115-02 |
| Immunogen: | Recombinant protein of human β-Actin |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB,1:10000 - 1:140000 IHC-P,1:500 - 1:1000 IF/ICC,1:200 - 1:800 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 42kDa |
| Purification: | Affinity purification |
| Form: | liquid |
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Applications
WB,1:10000 - 1:140000 IHC-P,1:500 - 1:1000 IF/ICC,1:200 - 1:800 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant protein of human β-Actin
