Immunological Sciences

V5 Tag

Western Blot Analysis of recombinant protein V5 protein with Ms Anti-V5 Tag At a dilution of 1:2,000 Secondary antibody HRP Goat Anti-Mouse IgG (H+L) at 1:10,0000 Blocking buffer: 3% notfat dry milk in TBST Det. ECL West Pico Plus – Exposure: 1s

Western Blot Analysis of recombinant protein V5 protein with Ms Anti-V5 Tag At a dilution of 1:2,000 Secondary antibody HRP Goat Anti-Mouse IgG (H+L) at 1:10,0000 Blocking buffer: 3% notfat dry milk in TBST Det. ECL West Pico Plus – Exposure: 1s

Catalog Number:MAB-91891
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/mL
Host:Ms
Isotype:IgG1
Clone:S6
Immunogen:A synthetic peptide of Human V5-Tag.
Reactivity:All Species
Applications:Western Blot: 1:500-1:5,000 Immunoprecipitation: 1:50-1:200 Immunofluorescence: 1:50-1:200
Purification:Purified
Form:Liquid

Applications

Western Blot: 1:500-1:5,000 Immunoprecipitation: 1:50-1:200 Immunofluorescence: 1:50-1:200

Immunogen

A synthetic peptide of Human V5-Tag.

Target Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Storage

Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term. Avoid repeated freeze and thaw cycles.

Buffer

In PBS (pH7.4), 0.02% Sodium Azide, and 50% glycerol pH7,3.

Western Blot Analysis of recombinant protein V5 protein with Ms Anti-V5 Tag At a dilution of 1:2,000 Secondary antibody HRP Goat Anti-Mouse IgG (H+L) at 1:10,0000 Blocking buffer: 3% notfat dry milk in TBST Det. ECL West Pico Plus – Exposure: 1s

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