Immunological Sciences

VEGFR2 / KDR

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:AB-84207
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human VEGF Receptor 2
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:240kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CD309;FLK1;VEGFR;VEGFR2;VEGF Receptor 2;KDR

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human VEGF Receptor 2

Target Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Synonyms:CD309;FLK1;VEGFR;VEGFR2;VEGF Receptor 2;KDR

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using VEGF Receptor 2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Immunohistochemistry of paraffin embedded rat testis using VEGF Receptor 2 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse testis using VEGF Receptor 2 antibody at dilution of 1:100 (40x lens).

No documents available.