Immunological Sciences

Vimentin Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-84199
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Vimentin. AA range:56-105
Reactivity:Hu, Ms
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.
Molecular Weight:50-57kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:CTRCT30;HEL113;Vimentin;VIM;vimentin

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.

Immunogen

The antiserum was produced against synthesized peptide derived from human Vimentin. AA range:56-105

Target Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract

Synonyms:CTRCT30;HEL113;Vimentin;VIM;vimentin

Storage

Store at -20℃. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECLWest Pico Plus. Exposure time: 30s.

Immunohistochemistry of paraffin embedded human lung cancer using Vimentin antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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