Vinculin Rabbit Polyclonal Antibody

Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane
on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.

Catalog Number:AB-83451
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Vinculin. AA range:786-835
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry (paraffin-embedded issues): 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000
Molecular Weight:123 kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry (paraffin-embedded issues): 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000

Immunogen

The antiserum was produced against synthesized peptide derived from human Vinculin. AA range:786-835

Target Background

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.

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