Immunological Sciences

Vinculin Rabbit Polyclonal Antibody

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Catalog Number:ABN00013
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:Recombinant protein of human Vinculin
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,ICC/IF 1:50-1:200,IP 1:20-1:50
Molecular Weight:Calculated MW: 124 kDa; Observed MW: 124 kDa
Purification:Affinity Chromatography
Form:Liquid
Synonyms:VCL; Vinculin; Metavinculin

Applications

WB 1:500-1:1000,ICC/IF 1:50-1:200,IP 1:20-1:50

Immunogen

Recombinant protein of human Vinculin

Target Background

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail. In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration.

Synonyms:VCL; Vinculin; Metavinculin

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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