Immunological Sciences

VPS35

Western blot analysis of extracts of various cell lines, using VPS35 antibody at 1:1000 dilution._ Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._ Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST._ Detection: ECL Enhanced Kit. Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using VPS35 antibody at 1:1000 dilution._ Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._ Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST._ Detection: ECL Enhanced Kit. Exposure time: 60s.

Catalog Number:AB-84318
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35.
Reactivity:Hu, Ms
Applications:WB 1:500 - 1:2000
Molecular Weight:92kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:VPS35;MEM3;PARK17

Applications

WB 1:500 - 1:2000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human VPS35.

Target Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Synonyms:VPS35;MEM3;PARK17

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using VPS35 antibody at 1:1000 dilution._ Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._ Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST._ Detection: ECL Enhanced Kit. Exposure time: 60s.

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