Immunological Sciences

ZAP-70 Rabbit Polyclonal Antibody

<p style="text-align: left;">Western blot analysis of lysates from Jurkat cells, using ZAP-70 Antibody. The lane on the right is blocked with the synthesized peptide.</p>

<p style="text-align: left;">Western blot analysis of lysates from Jurkat cells, using ZAP-70 Antibody. The lane on the right is blocked with the synthesized peptide.</p>

Catalog Number:AB-84734
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human ZAP-70. AA range:286-335
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000 ELISA: 1/10000
Molecular Weight:70kDa
Purification:Affinity purification
Form:Liquid
Synonyms:ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1/200 - 1/1000 ELISA: 1/10000

Immunogen

The antiserum was produced against synthesized peptide derived from human ZAP-70. AA range:286-335

Target Background

Zeta chain of T cell receptor associated protein kinase 70(ZAP70) Homo sapiens This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Synonyms:ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

<p style="text-align: left;">Western blot analysis of lysates from Jurkat cells, using ZAP-70 Antibody. The lane on the right is blocked with the synthesized peptide.</p>

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ZAP-70 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Jurkat cells using ZAP70 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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