ZNF281

Western blot analysis of extracts of 
mouse spleen, using ZNF281
 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus. 
Exposure time: 10s.

Western blot analysis of extracts of mouse spleen, using ZNF281 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-84194
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 676-895 of human ZNF281.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:110kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:ZNF281; ZBP-99; ZNP-99; zinc finger protein 281.

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 676-895 of human ZNF281.

Target Background

Synonyms:ZNF281; ZBP-99; ZNP-99; zinc finger protein 281.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of mouse spleen, using ZNF281 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of lysates from Hela, A549, MCF-7 cell line, mouse liver, mouse small intestine tissue lysate (from left to right), using ZNF281 Antibody (Center) ZNF281 was diluted at 1: 1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1: 10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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